TABLE 1.
PZA/5-ClPZA turnover and MICs in M. smegmatis mutants
| M. smegmatis strain | Description | PZA/5-Cl PZA turnover (nmol/min/ml of cells [OD600]) | MIC (μg/ml) |
Reference(s) | |
|---|---|---|---|---|---|
| 5-Cl PZA | PZA | ||||
| mc2155 | Wild-type strain | 0.85 ± 0.1 | 25 | >4,000 | 19, 24 |
| mc22612 | Spontaneous 5-Cl PZAr mutant | 32 ± 3 | 125 | >4,000 | 24 |
| mc27031 | mc2155 MSMEG_1088::magellan4 | 100 ± 15 | 125 | 150 | This work |
| mc27032 | mc2155 ΔMSMEG_1088 | 65 ± 5 | 125 | 150 | This work |
| mc27034 | mc27031 ΔMSMEG_1090 | 1.2 ± 0.1 | 25 | >4,000 | This work |
| mc27035 | mc2155 ΔMSMEG_1090 | 0.95 ± 0.15 | 25 | >4,000 | This work |
| mc27036 | mc22612 ΔMSMEG_1090 | 1.2 ± 0.2 | 25 | >4,000 | This work |
| mc27037 | Spontaneous 5-Cl PZAr mutant | 100 ± 15 | 125 | 150 | This work |
| mc27038 | mc2155 attBL5::PTc::pzaAMsmeg | 34 ± 1 | 125 | >4,000 | This work |