FIG. 9.

TprK lacks the amphiphilic character of β-barrel-forming proteins. (A) Freshly harvested T. pallidum, solubilized in 2% Triton X-114, was phase partitioned and subjected to immunoblot analysis with monospecific antisera directed against TrpK, TP0326, thioredoxin (Trx), and TroA. Lanes: whole cells (WC), aqueous phase (Aq), detergent-enriched phase (Det), and insoluble material (Ins). (B) Purified recombinant E. coli BamA and OmpG were phase partitioned and stained with Gel-code Blue following SDS-PAGE.