FIG. 5.

(A) Growth of 8325-4 and isogenic ΔgapA, ΔgapB, and ΔgapA ΔgapB mutants after 5 h of incubation in TM medium with varied concentrations of glucose. Cultures were grown at 37°C with aeration, and growth was measured as the optical density of the culture at 600 nm. Each experiment was repeated three times on different days, and results are presented as averages with error bars indicating the standard deviations. (B) Effect of the GAPDH mutations on the pathogenesis of S. aureus infection in G. mellonella. The graph indicates the percent viability of infected G. mellonella larvae at 24, 48, and 72 h postinfection with S. aureus 8325-4 wild-type strain and the isogenic ΔgapA, ΔgapB, and ΔgapA ΔgapB mutants. With the PBS negative control, larvae showed 100% survival at all time points. The results from three independent experiments were combined and used to produce survival curves using the Kaplan-Meier method.