TABLE 1.
Strain | Description | Reference or source |
---|---|---|
E. coli | ||
TOP10 | E. coli cloning strain | Invitrogen |
BL21 DE3 | IPTG-inducible T7 expression strain | 34 |
S. aureus | ||
RN4220 | Restriction deficient 8325-4 | Laboratory stock |
8325-4 | Wild-type 8325 cured of prophages | 15 |
8325-4 gapA strain | 8325-4 ΔgapA::tet | This study |
8325-4 gapB strain | 8325-4 ΔgapB::spc | This study |
8325-4 gapA gapB strain | 8325-4 ΔgapA::tet ΔgapB::spc | This study |
8325-4 gapR strain | 8325-4 ΔgapR::spc | This study |