FIG. 5.

(A) Principal-component analysis (PCA) representation of replicate rat gene expression profiles in the draining lymph node at 36 and 60 h after intradermal injection of sterile PBS, 10³ WT Y. pestis organisms, or 10⁸ pYV⁻ Y. pestis organisms. WT-infected samples were separated into two groups based on whether or not bacteria had disseminated from the lymph node to the blood: bubonic (sterile spleen) and septicemic (viable bacteria in the spleen). (B) Molecular and cellular function classification of genes whose expression was altered by infection with WT or pYV⁻ Y. pestis. The functions shown are the top three functional groups from the WT-infected samples. (C) Venn diagrams representing the numbers of rat genes upregulated or downregulated ≥2-fold in the lymph nodes of infected rats compared to uninfected control rats.