FIG. 11.

Macrophages derived from IL-17A^−/− mice, compared to those derived from wild-type mice, do not have a deficiency in killing C. neoformans strain H99 yeasts or in iNOS activity. Pulmonary leukocytes were isolated from lung tissues on day 7 postinoculation, and macrophages were enriched for by positive selection of CD11b⁺ cells. (A) Macrophages were grown in complete medium, cultured for 24 h, lysed, and subsequently plated on YPD agar for CFU determination. (B) Culture supernatants were also utilized to determine iNOS activity. Data represent cumulative results from two experiments utilizing four mice per group.