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. 2010 Sep 27;78(12):5074–5085. doi: 10.1128/IAI.00698-10

TABLE 1.

Primers used in real-time quantitative PCR for TLR amplification and detection

Gene Primer
Amplicon size (bp)
Forward Reverse
TLR1 CACAGCTCCTTGGTTTTAATG TGGGTATAGGACGTTTCTGTAG 102
TLR2 TGGAGCATCCGAATTGCATCACCG GAGCGGCCATCACACACCCC 193
TLR3 CGGGGGTCCAACTGGAGAACCT GGGCGTTGTTCAAGAGGAGGGC 198
TLR4 GCCTCCCTGGCTCCTGGCTA AGGGACTTTGCTGAGTTTCTGATCCA 139
TLR6 CCGTCAGTGCTGGAAATAG CGATGGGTTTTCTGTCTTGG 108
TLR9 CAGTTTGTCAGAGGGAGC ACTTCAGGAACAGCCAATTG 198
β-Actin CCAACCGTGAAAAGATGACC AGCATAGCCCTCGTAGATG 170
β2 m ATGGCTCGCTCGGTGACCCT TTCTCCGGTGGGTGGCGTGA 110