FIG. 2.

Incubation of enteric cocultures with secreted components from E. histolytica (Eh-SEC) caused damage to axonal structure and loss of neurons. (A) Representative image of fluorescence immunocytochemistry of a control coculture labeled with antibodies to HuD to identity neuronal cell bodies (green; e.g., arrowheads) and SNAP-25 to label axon structure (red; arrow). Hoechst 33342 was added to revel all nuclei (blue). Scale bar, 50 μm. (B) Image of coculture after treatment with 30 μg of Eh-SEC/ml for 2 days, with immunocytochemistry as in panel A. Note the disruption in neurite structure (circles). Scale bar, 50 μm.