FIG. 4.

Characterization of the effect of E. histolytica products on non-neuronal cells in coculture. (A) Glial cell number was unchanged in cocultures treated for 48 h with 30 μg of Eh-SEC or Eh-SOL (n = 4), relative to cohort cocultures treated with control vehicle. Glia were identified by GFAP immunocytochemistry. (B) Eh-SEC or Eh-SOL (30 μg/ml) addition to enteric cocultures did not significantly increase [³H]thymidine incorporation in intestinal smooth muscle cells. Rat serum (2.5% RS) was as a positive control (*, P < 0.05 versus control; n = 3).