FIG. 5.

Treatment of cocultures with amebic proteins results in a concentration-dependent reduction in neuronal content. The majority of the losses in neuron and axon number was achieved by 4 h postaddition and was not significantly increased by 48 h. (A) Quantification of the number of neurons and axons present in cocultures treated with Eh-SEC or Eh-SOL for 4 h. In all cases, there was a significant decrease in neuron and axon number with either treatment relative to control (n = 4; P < 0.05). (B and C) Concentration-dependent decrease in the number of neurons (B) and axons (C) after 48 h of incubation with Eh-SEC or Eh-SOL (n = 3 to 13; *, P < 0.05).