FIG. 1.

Native and recombinant PyMIF. (A and B) The expression of the pymif gene was measured by quantitative RT-PCR in P. yoelii 17X and P. yoelii 17XL blood-stage parasites (A) and in the ring, trophozoite, and schizont stages of P. yoelii 17X (B), isolated by density gradient centrifugation. pymif mRNA levels are expressed relative to those of PY07409, a 60S P. yoelii ribosomal protein gene. All reactions were carried out in triplicate. (C) Coomassie blue-stained SDS-polyacrylamide gel containing E. coli lysates of uninduced (lane 2) and induced (lane 3) cells expressing rPyMIF and purified rPyMIF (lane 4). (D) Immunoprecipitation of metabolically labeled P. yoelii proteins released into culture supernatants (S) or remaining parasite associated (P), using anti-rPyMIF and normal mouse sera.