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. 2010 Sep 13;78(12):5151–5162. doi: 10.1128/IAI.00277-10

FIG. 2.

FIG. 2.

(A) Mouse anti-rPyMIF antibodies do not recognize mammalian MIF. Immunoblots of 15% polyacrylamide gels containing rPyMIF (25 ng) and recombinant human MIF (100 ng) were probed with anti-rPyMIF sera or normal mouse sera (NMS) diluted 1:1,000. Bound antibody was detected by chemiluminescence using rabbit anti-mouse IgG conjugated to horseradish peroxidase (1:10,000). (B) Localization of PyMIF in P. yoelii-infected erythrocytes. PyMIF was detected in parasitized erythrocytes by indirect immunofluorescence using P. yoelii 17XL parasites fixed in 4% formaldehyde and 0.0075% glutaraldehyde using anti-rPyMIF (top row) or normal mouse (bottom row) sera. Bound antibody was detected with goat anti-mouse IgG conjugated to FITC. Parasite nuclei were stained with DAPI.