FIG. 5.

Elevated expression of PyMIF in Py17X-MIF(+) transgenic parasites. Lysates of P. yoelii 17X (wild type [WT]) and Py17X-MIF(+) (transgenic [Tg]) blood-stage parasites were separated by SDS-PAGE, and the levels of PyMIF were compared by immunoblot analysis. Blots were probed with rPyMIF-specific polyclonal sera (1:2,000) or a pool of NMS. The total amount of parasite protein loaded per lane (6 μg) was normalized based on the reactivity of rabbit anti-PyMSP8 on parallel immunoblots. The expression profile of PyMSP8 across blood-stage malaria parasites (53) was similar to that observed for endogenous PyMIF.