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. 2010 Sep 13;78(12):5151–5162. doi: 10.1128/IAI.00277-10

FIG. 8.

FIG. 8.

Elevated expression of PyMIF in Py17XL-MIF(+) transgenic parasites. Lysates of P. yoelii 17XL (WT) and Py17XL-MIF(+) (Tg) blood-stage parasites were separated by SDS-PAGE, and the levels of PyMIF were compared by immunoblot analysis. Blots were probed with rPyMIF-specific polyclonal sera (1:2,000) or a pool of NMS. The total amount of parasite protein loaded per lane (3 μg) was normalized based on the reactivity of rabbit anti-PyMSP8 on parallel immunoblots. The expression of PyMSP8 across blood-stage malaria parasites (53) was similar to that observed for endogenous PyMIF.