FIG. 2.

Flow cytometry to detect antimycobacterial CD4⁺, CD8⁺, and γδ TCR⁺ T cells and suppression by 3/46 supernatants. (A) CFSE-labeled PBMC were cultured in RPMI complete medium (left), in control 3/28 SN cells infected with BCG (middle), or in cells from 3/46 SN and infected with BCG (right). (B) Responding CD3⁺ CD4⁺, CD3⁺ CD8⁺, or CD3⁺ γδ⁺ T cells were enumerated by determining absolute numbers of CFSE^lo IFN-γ⁺ T cells after 7 days in culture. Bar graphs indicate the medians of 14 experiments. Asterisks indicate significant differences from T cell responses in 3/28 SN (P < 0.002 for all T cell subsets by Wilcoxon matched pairs test).