FIG. 8.

Analysis of OmpX localization in E. coli K-12 D21 strains. OmpX-expressing E. coli D21, D21e7, D21f1, and D21f2 and their parental strains were grown to mid-exponential phase at 37°C and collected by centrifugation. Whole-cell lysate proteins (A) and Triton X-100-insoluble (B) or 5 M urea-insoluble (C) outer membrane proteins were extracted, separated by SDS-PAGE, and stained with Coomassie blue. Molecular mass markers were estimated with a prestained Pageruler protein standard (Fermentas). Arrows indicate OmpX protein.