FIG. 6.

Analysis of needle-associated proteins within ipaB internal deletion mutants. (A) Overnight total cultures from the wild type (WT), ΔipaB, ipaB internal deletion mutants, and ΔipaD-overexpressing MxiH were analyzed by immunoblotting with anti-MxiG and anti-MxiH antibodies. (B) Needles were purified and normalized for the amount of MxiH by silver-stained SDS-PAGE (bottom) and analyzed by Western blotting. The antibodies used for the blots are indicated on the right. The data shown are representative of 2 independent assays giving similar results.