TABLE 2.
Primer sequences used in this study
| Primer | Sequencea |
|---|---|
| ipaB-F | 5′-GGGGAAGCTTGATGCATAATGTAAGCACCACAAC-3′ |
| ipaB-R | 5′-GGGGCTGCAGTCCTTATTTGTATCAAGCAGTAGT-3′ |
| d167-176b | 5′-CCAAATTCAAACAAGATTATCCAAATTAAGCCGGGAAGAAATAC-3′ |
| d227-236b | 5′-GAAATAGACTCTTTTTCTGCATCAACCCAGCAGAAATCATTAAC-3′ |
| d257-266b | 5′-CTCAATTGATGGCAACCTTTTCTTTAAAAAATGATCTGGC-3′ |
| d297-306b | 5′-CTGATGAGTATGCTGCTGAAATGGGTTGTGTTGGGAAAATAC-3′ |
| d307-316b | 5′-GCAGAAGAACTCAACAGAGTACTTTTAACTATCGTTAGT-3′ |
| d325-334b | 5′-CTATCGTTAGTGTTGTTGCACTGGCAGCTGTTGGTTTAGC-3′ |
| d410-417b | 5′-GGGGCAATCGCAGGCGCTCTCGTAGCCACTGTTGG-3′ |
a
Underlined letters represent non-ipaB sequences and restriction endonuclease sites generated to facilitate cloning mutants.
b
These primers were paired with ipaB-R to amplify the 3′ fragment of ipaB, and their reverse complementary sequences, which are not listed, were paired with ipaB-F to amplify the 5′ fragment of ipaB.