TABLE 3.
Summary of results obtained with this study
| Strain | Expressiona |
Constitutive secretiona | CR responsivenessa | % adhesion | % invasionc | % hemolysis | Membrane insertion | Tip composition | Mutant classb | |
|---|---|---|---|---|---|---|---|---|---|---|
| IpaB | IpaH | |||||||||
| WT | + | − | − | + | 22 ± 5.9 | 100 | 82.1 ± 4.0 | IpaBC | IpaDB(C) | NA |
| ΔipaB | − | +++ | + | − | 130 ± 31.1 | 0.3 ± 0.2 | 1.1 ± 1.7 | IpaD(C) | NA | |
| ΔipaB complemented | + | − | − | + | 11 ± 2.0 | 197.5 ± 21.2 | 72.9 ± 10.3 | IpaBC | IpaDB(C) | NA |
| ipaBΔ167-176 | ± | − | − | + | 15 ± 5.6 | 129.8 ± 33.5 | 45.0 ± 16.0 | IpaBC | IpaDB(C) | 1a |
| ipaBΔ227-236 | + | ++ | + | − | 164 ± 41.8 | 76.9 ± 17.5 | 13.7 ± 9.1 | IpaBC | IpaD(C) | 2a |
| ipaBΔ257-266 | ± | ++ | + | − | 160 ± 14.4 | 0.1 ± 0.1 | 0.9 ± 2.0 | IpaD(C) | 3 | |
| ipaBΔ297-306 | + | + | + | − | 213 ± 98.6 | 116.0 ± 63.4 | 2.9 ± 3.6 | IpaD(C) | 2a | |
| ipaBΔ307-316 | + | − | ± | + | 14 ± 6.9 | 7.7 ± 2.1 | 1.4 ± 2.6 | IpaDB(C) | 1b | |
| ipaBΔ325-334 | + | − | − | + | 14 ± 10.5 | 68.0 ± 35.9 | 33.4 ± 18.0 | IpaBC | IpaDB(C) | 1a |
| ipaBΔ410-417 | + | ++ | + | − | 342 ± 88.6 | 11.6 ± 1.2 | 5.8 ± 3.5 | IpaC | IpaD(C) | 2b |
a
Results are succinctly described as normal or positive (+), intermediate (±), and negative (−); refer to the text and corresponding figures for details.
b
NA, not applicable.
c
Normalized to adhesion.