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Parachlamydia acanthamoebae is internalized by macrophages. Intracellular detection of P. acanthamoebae within wild-type (A), TLR2−/− (B), and TLR4−/− (C) BMDMs at 4 h postinfection is shown. Macrophages and P. acanthamoebae were stained with Evans blue and Alexa 488, respectively, and analyzed by confocal microscopy as described in Materials and Methods.
