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Heat-inactivated Parachlamydia acanthamoebae stimulates cytokine production by RAW 264.7, J774, and peritoneal macrophages. TNF and IL-6 production by RAW 264.7 (A), J774 (B), and thioglycolate-elicited peritoneal (C) macrophages cultured with PBS, LPS, and living (Pac) or heat-inactivated (HI Pac) P. acanthamoebae is shown. Data are means ± SD for triplicates samples from one experiment; P < 0.05 for living versus heat-inactivated P. acanthamoebae (A to C).
