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Enhancement of Lyn expression in HL-60 cells decreases miR181b expression and increases Mcl-1 expression. A and B, 1 × 106 HL-60 cells were treated with 10 ng/μl 1,25-D3 for the indicated time. A, total RNA was extracted from cells, and miR181b expression was measured. Data represent the mean ± S.E.M. of triplicate samples. B, cells were lysed, and Western blot analysis was performed using the indicated antibodies. Representative data are shown.
