Effect of morphine on the expression of miRNA-133b in mammalian neurons. The level of miRNA-133b in 1-week (A) and 3-week (B) hippocampal neuron culture treated with 100 nM morphine, 1 μM naloxone, or morphine and naloxone simultaneously were determined by qRT-PCR. *, P ≤ 0.05; **, P ≤ 0.005 (unpaired Student's t test with Welch correction), n = 4.