Table 2.
Conjunctival Cells
| |
CRP (pg/mL) |
IL-1 (pg/mL) |
IL-10 (pg/mL) |
IL-12 (pg/mL) |
TNF-α (pg/mL) |
H2O2 (mg/mL) |
|---|---|---|---|---|---|---|
| Average ± σ | Average ± σ | Average ± σ | Average ± σ | Average ± σ | Average ± σ | |
| Media | 0.26 ± 0.06 | 0.01 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 |
| BAK 0.1000% | 13.15 ± 0.77 | 145.33 ± 5.69 | 19.90 ± 0.41 | 16.29 ± 0.71 | 316.44 ± 12.30 | 6.5 ± 1.64 |
| BAK 0.0750% | 10.16 ± 0.72 | 70.63 ± 3.27 | 3.14 ± 0.17 | 11.78 ± 0.54 | 123.23 ± 3.73 | 5 ± 0.00 |
| BAK 0.0500% | 9.31 ± 0.52 | 29.90 ± 1.90 | 2.28 ± 0.26 | 8.25 ± 0.43 | 32.19 ± 3.06 | 3 ± 0.00 |
| BAK 0.0250% | 7.65 ± 0.62 | 8.27 ± 0.61 | 1.05 ± 0.11 | 4.87 ± 0.30 | 6.80 ± 0.36 | 3 ± 0.00 |
| BAK 0.0200% | 6.32 ± 1.32 | 6.13 ± 0.79 | 0.94 ± 0.10 | 3.95 ± 0.31 | 3.93 ± 0.14 | 3 ± 0.00 |
| BAK 0.0150% | 7.11 ± 0.38 | 3.62 ± 0.26 | 0.70 ± 0.10 | 3.16 ± 0.21 | 2.07 ± 0.15 | 1 ± 0.00 |
| BAK 0.0100% | 6.83 ± 0.32 | 2.59 ± 0.33 | 0.60 ± 0.11 | 2.49 ± 0.17 | 1.15 ± 0.09 | 1 ± 0.00 |
| BAK 0.0075% | 4.98 ± 0.31 | 0.70 ± 0.03 | 0.21 ± 0.03 | 1.22 ± 0.22 | 0.12 ± 0.01 | 0.5 ± 0.55 |
| BAK 0.0050% | 3.49 ± 0.21 | 0.09 ± 0.01 | 0.07 ± 0.02 | 0.44 ± 0.07 | 0.01 ± 0.00 | 0.05 ± 0.55 |
| BAK 0.0025% | 2.26 ± 0.11 | 0.04 ± 0.00 | 0.01 ± 0.00 | 0.29 ± 0.05 | 0.00 ± 0.00 | 0 ± 0.00 |
| BAK 0.0010% | 1.74 ± 0.12 | 0.02 ± 0.00 | 0.00 ± 0.00 | 0.01 ± 0.00 | 0.00 ± 0.00 | 0 ± 0.00 |
| H2O2 0.1000% | 3.08 ± 0.14 | 9.28 ± 0.89 | 7.07 ± 0.39 | 4.59 ± 0.26 | 1.50 ± 0.07 | 80 ± 0.00 |
| H2O2 0.0750% | 1.12 ± 0.08 | 5.84 ± 0.51 | 3.01 ± 0.18 | 2.53 ± 0.21 | 0.30 ± 0.03 | 70 ± 5.16 |
| H2O2 0.0500% | 1.01 ± 0.07 | 2.65 ± 0.36 | 0.67 ± 0.12 | 0.72 ± 0.12 | 0.03 ± 0.01 | 50 ± 5.16 |
| H2O2 0.0250% | 0.94 ± 0.04 | 0.68 ± 0.08 | 0.12 ± 0.01 | 0.40 ± 0.05 | 0.00 ± 0.00 | 15 ± 5.89 |
| H2O2 0.0100% | 0.79 ± 0.03 | 0.26 ± 0.03 | 0.00 ± 0.00 | 0.16 ± 0.03 | 0.00 ± 0.00 | 5 ± 0.00 |
Concentrations of inflammatory biomarkers [C reactive protein (CRP), interleukin (IL)-1, interleukin (IL)-10, interleukin (IL)-12, and tumor necrosis factor (TNF)] elaborated by conjunctival epithelial cells (CCC: Wong-Kilbourne derivative of conjunctiva) after being put in contact with varying concentrations of each of the initial experimental testing solutions [media alone, benzalkonium chloride (BAK: 0.001%–0.1%), and hydrogen peroxide (H2O2: 0.01%–0.1%) for 1 h.