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. 2010 Oct 21;4:165. doi: 10.3389/fnins.2010.00165

Figure 10.

Figure 10

SD-induced transcripts in the neocortex label overlapping populations of excitatory neurons. (A–C) Double fluorescent ISH for Arc (green) and Gad1 (red) at low magnification (A) and high magnification in agranular insular cortex (B) and visual cortex (C) corresponding to boxed regions in (A), demonstrating lack of co-labeling in the neocortex. (D–S) Cellular colocalization of genes showing cortical gene upregulation following SD with canonical inhibitory (Gad1) and excitatory (Slc17a7) neuronal markers and with one another in agranular insular cortex (D,F,H,J,L,N,P,R) and primary visual cortex (E,G,I,K,M,O,Q,S). Fos is largely restricted to excitatory neurons (D–G), neither Nr4a1 nor Nptx2 colocalize with Gad1 (H–K), and Rasd1 partially overlaps with Gad1 (L,M). Arc, Nr4a1 and Fos label partially overlapping neuronal populations (N–S). (T–X) Gene-specific cellular specificity in layer 2 of somatosensory cortex following SD. Ccrn4l is expressed in a subset of Nr4a1 and Nptx2-positive excitatory neurons (T–V),Fos partially overlaps with Nptx2 (W), yet Ccrn4l and Fos label nearly non-overlapping populations (X). Numbers in (B–S) delineate cortical layers. Scale bars: (A), 1 mm; (B–S), 250 μm; (T–X), 100 μm.