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. 2010 Oct 21;4:165. doi: 10.3389/fnins.2010.00165

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Copyright © 2010 Thompson, Wisor, Lee, Pathak, Gerashchenko, Smith, Fischer, Kuan, Sunkin, Ng, Lau, Hawrylycz, Jones, Kilduff and Lein.

This is an open-access article subject to an exclusive license agreement between the authors and the Frontiers Research Foundation, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are credited.

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Difference grid analysis identified decreased Fos expression in the ventral posteromedial nucleus of the thalamus during SD. Difference grid representation of upregulated Fos expression in SDC versus SD, showing higher expression (orange) in SDC than SD in VPM at low magnification (A) and high magnification (C). Corresponding reference atlas panel is shown in (B). (D–F) Colorimetric Fos ISH demonstrating differential Fos mRNA localization in SD, with lowest levels in VPM in SD relative to all other conditions, with higher SD expression in surrounding thalamic areas, for three replicate experiments.