Fig. 1.

Agonist-induced trafficking of βarr2-GFP in U2OS cells containing GPR35a. A, representative images show no βarrestin2-GFP translocation at basal condition (HBSS), moderate response at 100 nM pamoic acid (PA), and strong response at 1 μM PA. Scale bar, 10 μm. B, the concentration response-curves are from images acquired in triplicate in three or more independent experiments and are computed by analyzing the images for the number of translocated βarrestin2-GFP aggregates (n = 4 for pamoic acid; n = 3 for zaprinast and kynurenic acid; n = 5 for NPPB).