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Agonist-mediated GPR35a internalization in U2OS Cells. A, vehicle-treated (HBSS) cells prelabeled with anti-HA mouse antibody to GPR35a show predominantly plasma membrane receptor staining. Ten micromolar pamoic acid induces internalization of membrane bound GPR35a forming aggregates in the cytosol. Scale bar, 10 μm. B, quantification of GPR35a receptor internalization by pamoic acid and zaprinast using on-cell Western analysis with a LI-COR Odyssey IR imager (n = 4).
