Fig. 5.

Agonist-mediated ERK1/2 phosphorylation in U2OS cells expressing GPR35a. A, concentration response of ERK1/2 phosphorylation of GPR35a cells treated for 15 min with pamoic acid. B, in-cell Western analysis with a LI-COR Odyssey IR imager using 96-well plate. C, response curves of ERK1/2 phosphorylation from in-cell Western analysis treated with pamoic acid (left) or zaprinast (right) (n = 3). Pretreatment of UGPR35β cells with 200 ng/ml PTX for 3 h results in blockade of the ERK response.