Fig. 6.

Compound CID2745687 mediated antagonism of βarr2-GFP response and ERK1/2 phosphorylation. UGPR35β cells were imaged after 40-min incubation of combinations of compounds. A, antagonism of βarr2-GFP response with CID2745684 and CID2745687. B, representative images of 1 μM compound CID2745687 alone (left), 1 μM pamoic acid alone (middle), and 1 μM CID2745687 coapplied with 1 μM pamoic acid (right). Scale bar, 10 μm. C, inhibition of βarr2-GFP redistribution in the presence of 1 μM pamoic acid by CID2745687 is dose-dependent; n = 3 (left) and reversible; n = 3 (right). D, inhibition of ERK1/2 phosphorylation in the presence of 1 μM pamoic acid by CID2745687 is dose-dependent; n = 3 (left) and competitive; n = 3 (right).