Figure 4.

oxidative metabolism and AMPK activity. (A) Rate of palmitate oxidation in isolated working hearts from the adult TMPG and TMCM mice14 days after the end of tamoxifen treatment were measured using ³H labeled palmitate. Data are expressed as mean±SEM (n=6, *p<0.05). (B) Rate of glucose oxidation in isolated working hearts from the adult TMPG and TMCM mice 14 days after the end of tamoxifen treatment were measured using ¹⁴C labeled glucose. Data are expressed as mean±SEM (n=6, p>0.05). (C) Myocardial oxygen consumption was measured during the isolated heart perfusion. Data are expressed as mean±SEM (n=6, *P<0.05). (D) Western blot analysis of phosphory-lated AMPKα and AMPKα. AMPK activity was estimated by the ratio of pAMPKα to AMPKα. Data are expressed as mean±SEM (n=4, *P<0.05).