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. Author manuscript; available in PMC: 2010 Nov 15.
Published in final edited form as: J Cell Physiol. 2009 May;219(2):438–448. doi: 10.1002/jcp.21687

Fig. 7.

Fig. 7

Rescue of HiNF-P co-activation by p57KIP2 siRNA and changes in endogenous histone H4 gene expression upon p57KIP2 modulation. Treatment of HeLa cells that express endogenous p57KIP2 with siRNA specific for p57KIP2 restores co-activation of HiNF-P and p220NPAT. HeLa cells were transfected with the H4 promoter-luciferase reporters in the presence (+) or absence of p220NPAT and HiNF-P expression vectors. After ~12–16 h, cells were treated with (+) control siRNA or p57KIP2 siRNA (100 nM). Luciferase activity was measured after 48 h. Selective siRNA mediated deficiency of p57KIP2 relative to Cyclin E, CDK2 and HiNF-P was confirmed by immunoblotting.