Fig. 1. siRNA knockdown of EWS-Fli1 affects gene expression in Ewing cells.
(A) SK-ES cells were transfected with a synthetic siRNA against EWS-Fli1 or a control non-specific siRNA against firefly luciferase (lanes 1–4). 3 days after transfection, cells lystes were blotted with a rabbit polyclonal anti-Fli1 antibody or a mouse monoclonal anti-β-actin. (B) After transfection with a synthetic siRNA against EWS-Fli1, gene expression in these SK-ES cells was analyzed by DNA microarray (in duplicates) and normalized to SK-ES cells transfected with a control siRNA against firefly luciferase. Only genes significantly affected by EWS-Fli1 knockdown are listed.