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. Author manuscript; available in PMC: 2011 Nov 5.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Oct 8;402(1):129–134. doi: 10.1016/j.bbrc.2010.09.129

Fig. 3. Induction of FOXO1 by EWS-Fli1 knockdown.

Fig. 3

(A) siRNA against EWS-Fli1 were turned on in three Ewing cell lines harboring the pSLIK-Neo-siEWS-Fli1 vector, and RNAs were collected for RT-PCR analysis. (B) Lysates from these three Ewing cell lines were blotted to show induction of FOXO1 protein after EWS-Fli1 knockdown. (C). pFHRE-Luc reporter was transfected into A673 cells harboring siEWS-Fli1 or a scrambled siRNA. The transfected cells were then cultured in medium with or without doxycycline for 4 days, and luciferase activities were measured and normalized to the pRL-SV40 controls.