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. Author manuscript; available in PMC: 2011 Nov 5.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Oct 8;402(1):129–134. doi: 10.1016/j.bbrc.2010.09.129

Fig. 4. Direct binding of EWS-Fli1 to FOXO1 promoter.

Fig. 4

(A) Western blotting was carried out using A673 cells after 3-day treatment with doxycycline to induce siRNA production. (B) The luciferase reporter plasmids were transfected into A673 cells harboring inducible siRNA against EWS-Fli1, treated with or without doxycycline for 3 days before luciferase assay. (C) CHIP assay and PCR were performed with fragmented chromatin from A673 cells following doxycycline-induced EWS-Fli1 knockdown.