Figure 7.

Granulysin released from NK cells upon degranulation activates DCs. Mo-iDCs were cultured at 1 × 10⁶ cells/mL in the presence or absence of concentrated supernatants (10% of final volume) from SrCl₂-treated NK92 (A) or primary NK cells (B) for 48 hours. Ultrapure LPS (1 μg/mL) was used a positive control. Cells were then analyzed for expression of costimulatory molecules on Mo-DCs by FACS (A) and production of cytokines by Mo-DC (B). For panel A, shaded gray area represents isotype control. Data are shown as geometric mean fluorescence of intensity. One representative experiment of 2 is shown.