Fig. 2.

Flowchart for targeted comparisons of genomic sequences. BAC libraries are used to isolate orthologous regions of interest from the genomes of two species (1, 2). Sequences are obtained from the respective BAC clones (3) and aligned for comparison (4). Testable hypotheses derived from the sequence comparisons, such as putative regulation of gene expression by conserved DNA sequence elements, are then analyzed by functional experiments in vivo and in vitro. See Miyake and Amemiya (2004) and Amemiya and Gomez-Chiarri (2006) for in-depth reviews of the comparative genomics pipeline. Modified from Miyake and Amemiya (2004) with permission. Copyright (2004) Elsevier, Inc.