Table 1.
Summary statistics for the notothenioid BAC libraries
| Chaenocephalus aceratus (VMRC-21) | Notothenia coriiceps (VMRC-19) | |
|---|---|---|
| Vector used | pCC1BAC | pCC1BAC |
| Restriction enzyme used | EcoRI | EcoRI |
| DNA source | Leucocytes | Erythrocytes |
| Number of 384-well plates | 336 | 288 |
| Eschericia coli strain used | DH10B T1 resistant | DH10B T1 resistant |
| Empty wells | 0 | 0 |
| Estimated non-recombinant clones and vector artifact clones, % | 6451 (5) | 8847 (8) |
| Estimated insert-containing clones, % | 122 573 (95) | 101 745 (92) |
| Average insert size | 168 kb | 138 kb |
| Median insert size | 168 kb | 155 kb |
| Estimated genome coverage | 10X | 12X |
| Restriction enzyme used | EcoRI | EcoRI |
Reproduced from Detrich et al. (2010). Copyright (2010 Wiley-Liss, Inc. a subsidiary of John Wiley & Sons, Inc.); Reprinted with permission of John Wiley & Sons, Inc.