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. Author manuscript; available in PMC: 2011 Nov 19.
Published in final edited form as: FEBS Lett. 2010 Oct 21;584(22):4619–4622. doi: 10.1016/j.febslet.2010.10.029

Fig 4.

Fig 4

Recombinant WT and mutant Cu+-ATPase. Current transients induced by 100μM ATP concentration jumps in the presence of 5μM CuCl2 (solid lines) or 1mM BCS (dotted lines) on WT and mutant Cu+-ATPase: (A) WT Cu+-ATPase, (B) C983A/C985A mutant, and (C) C575A/C578A mutant. The right upper corner inset shows Western blots of the WT and two mutant proteins demonstrating nearly identical expression levels. The Western blots were obtained with an antibody reacting with the same c-Myc tag present in the three constructs.