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. Author manuscript; available in PMC: 2011 Nov 15.
Published in final edited form as: Chem Res Toxicol. 2010 Sep 1;23(11):1714–1725. doi: 10.1021/tx100186t

Figure 2.

Figure 2

Analysis of the enzymatic hydrolysate of NVP-modified Hb, obtained upon reaction with 12-mesyloxy-NVP and subsequent hydrolysis with pronase E and leucine aminopeptidase M. (a) HPLC-ESI/MS total ion chromatogram; (b) extracted HPLC-ESI/MS ion chromatogram and mass spectrum of the m/z 370 ion, presumed to be a serine adduct; (c) HPLC-ESI-MS/MS ion chromatograms and product ion mass spectra of the m/z 386 and 469 ions; (d)-(e) HPLC-ESI-MS/MS ion chromatograms and product ion mass spectra of the NVP adduct standards with (d) cysteine, and (e) tryptophan.