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Detection and location of NVP-modified peptides. The panels show representative sections of the MALDI-TOF spectra of tryptic digests of (a) unmodified and NVP-modified HSA, and (b) unmodified and NVP-modified Hb. New m/z peaks, absent from the controls, are clearly detected in the mass spectra of the NVP-modified proteins (highlighted in the figure). These new m/z values correspond to the mass of a peptide from HSA or Hb plus the mass increment characteristic of NVP modification.
