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. Author manuscript; available in PMC: 2011 Nov 12.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Oct 8;402(2):241–246. doi: 10.1016/j.bbrc.2010.10.006

Fig. 2.

Fig. 2

Expression of NR1a/NR2B receptors in Xenopus oocytes and activation of NR1a/NR2B receptors by ACM(+). A. Expression of NR1/NR2B on Xenopus oocyte membranes after microinjection of a mixture of NR1 and NR2B cRNAs. Left lane: NR2B subunit expression in the NR1a/NR2B mRNA-injected oocytes. Note a clear band with an approximate molecular weight of 180 kD in the left lane. Result at right lane was from non-injected control oocytes, showing that no recognizable band was detected. B. An example of inward currents induced by IL-1β-stimulated ACM (ACM+). The results on the left column were from two non-injected oocytes and the data on the right column were from two different oocytes injected with NR1a/NR2B cRNAs. Note that ACM(+) produced an inward current in NR1a/NR2B-expressing oocytes, but not in non-NR1a/NR2B-expressing oocytes (non-NR1a/NR2B injected). Application of ACM(-) had no apparent effects in producing inward current. These results suggest that IL-1β-stimulated astrocytes secrete NMDA receptor agonist-like substances, resulting in activation of NR1a/NR2B receptors expressed in Xenopus oocytes.