Table 3.

CD4⁺ T-cell responses of immunized mice the day before the i.n. challenge with C. trachomatis MoPn^a

Groups	CD4+ cell proliferative response to EBb (103 × Δcpm)	In vitro cytokine production in response to EB (pg/ml)
		IFN-γ	IL-4
rMOMP- s.l.	4.6 ± 2.1c	73 ± 2c,d	< 4
rMOMP- s.l.+i.m.+s.c.	7.6 ± 2.2c	93 ± 0c,e,f,g	< 4
rMOMP- c.l.	8.5 ± 3.8c	48 ± 1c	< 4
rMOMP- c.l.+i.m.+s.c.	5.8 ± 3.1c	78 ± 6c,i	< 4
rMOMP- i.m.+s.c.	10.8 ± 6.1c	83 ± 2c	< 4
Ng-rPorB- i.m.+s.c.	1.6 ± 0.5	< 31	< 4
MoPn- i.n.	18.7 ± 5.5c	1,659 ± 110c	< 4

^aValues are mean ± 1SD.

^bC. trachomatis MoPn EB were added at a ratio of EB : APC+CD4⁺ cells of 1:5.

^cP < 0.05 by the Student’s t test in comparison with Ng-rPorB immunized group.

^dP <0.05 by the Student’s t test by comparing rMOMP- s.l. group with rMOMP- c.l. group.

^eP <0.05 by the Student’s t test by comparing rMOMP- s.l.+i.m.+s.c. group with rMOMP- i.m.+s.c. group.

^fP < 0.05 by the Student’s t test by comparing rMOMP- s.l.+i.m.+s.c. group with rMOMP- s.l. group.

^gP <0.05 by the Student’s t test by comparing rMOMP- s.l.+i.m.+s.c. group with rMOMP- c.l.+i.m.+s.c. group.

^hP < 0.05 by the Student’s t test by comparing rMOMP- c.l.+i.m.+s.c. group with rMOMP- c.l. group.