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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Glia. 2010 Sep 27;59(1):1–13. doi: 10.1002/glia.21071

Figure 5. BzATP-mediated Egr target gene induction is dependent on the P2X7-Egr pathway.

Figure 5

Parental N9, shEgr/N9 or shP2X7/N9 microglia were treated with either vehicle (250 mM Hepes) or BzATP (150μM) for 1 hour (A, B) or 20 hours (C). (A) BzATP-induced Egr expression is blunted in shEgr/N9 cells. (B) RNAi to P2X7 and Egr factors prevents BzATP-induced Egr target gene (Nab-1/Nab-2) activation. Nab gene induction by BzATP in parental cells ranged from 3-14 fold over vehicle treatment. (C) RNAi to Egr factors, but not to P2X7 receptors, decreases VEGF expression. The data are graphed as fold induction relative to BzATP-treated parental N9 cells for each gene and represent the means of 4-8 independent experiments + SEM.