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. Author manuscript; available in PMC: 2011 Nov 12.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Oct 12;402(2):351–355. doi: 10.1016/j.bbrc.2010.10.032

Figure 3.

Figure 3

TpbB enzyme activity as a DGC. (A) Domain organization of TpbB. Two transmembrane domains, the HAMP domain and the GGDEF domain are indicated. (B) Representative 31P NMR spectrum after converting GTP to c-di-GMP by TpbB. (C) Production of c-di-GMP by TpbB from 5 mM GTP; the c-di-GMP concentration was calculated according to the peak integration value in the 31P NMR spectrum. The experiment was performed twice, and the dashed line indicates feedback inhibition for c-di-GMP concentrations higher than 0.2 mM.