. Author manuscript; available in PMC: 2011 Nov 12.

Published in final edited form as: Biochem Biophys Res Commun. 2010 Oct 12;402(2):351–355. doi: 10.1016/j.bbrc.2010.10.032

Table 1.

Strains and plasmids used in this study. Gm^R, Km^R, Car^R, and Ap^R indicate gentamicin, kanamycin, carbenicillin, and ampicillin resistance, respectively.

Strains and plasmids	Relevant genotype	Source
*P. aeruginosa*
PA14	Wild-type strain	[9]
tpbA	PA14_13660 Ω Mar2xT7, Gm^R	[9]
*E. coli*
BL21(DE3)	F⁻ompT hsdS_B(r_B⁻m_B⁻) gal dcm λ(DE3) Ω placUV5∷ T7 polymerase	Novagen
Plasmid
pMQ70-tpbB	P_BAD∷tpbB, complementation plasmid, Car^R, Ap^R	[4]
pET28b-tpbA	P_T7∷tpbA-cHis⁺, expression vector for TpbA-cHis, Km^R	[4]