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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Eur J Neurosci. 2010 Oct 21;32(10):1618–1631. doi: 10.1111/j.1460-9568.2010.07447.x

Fig. 6. Mouse L1-CAM (mL1CAM) axonal targeting is regulated by protein phosphorylation and induces axonal bundling.

Fig. 6

GFP was fused to the C-terminus of mL1CAM (mL1CAM-GFP). (A) When expressed in cultured hippocampal neurons, mL1CAM-GFP (green) mainly localized in axons. Dendrites were revealed with the MAP2 staining (red). (B) After treated with Stau, mL1CAM-GFP was mainly localized in dendrites but not axons. (C) GFP fluorescence levels of control (A) and Stau-treated neurons (B) along the axon (blue) and two main dendrites (red and dark red). Background value, 230, was subtracted. (D) mL1CAM-GFP-expressing axons formed bundles with NgCAM-mCherry-expressing axons. Arrows, axons. Arrowheads, dendrites. Scale bars, 100 μm.