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. Author manuscript; available in PMC: 2011 Nov 15.
Published in final edited form as: Chem Res Toxicol. 2010 Oct 8;23(11):1656–1662. doi: 10.1021/tx100192f

Figure 3.

Figure 3

Representative two-dimensional gel maps of overall protein expression in SH-SY5Ycells. Protein expression from (A) cytosolic fraction, (B) membrane fraction, (C) Nuclear fraction, and (D) cytoskeletal fractions are shown. The majority of the proteins are located between 20 and 120 kDa, and are widely distributed across the entire pI range (pI: 3–10). Many proteins are identified as multiple spots in a single fraction, suggesting possible post-translational modifications such as glycosylation or phosphorylation. Circled spots displayed an expression change of ±1.3 fold or greater. Protein spots were excised from the gel and identified by MALDI-TOF mass spectrometry and a peptide mass fingerprint search.