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. 2010 Nov 5;2(4):250–255. doi: 10.4055/cios.2010.2.4.250

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Copyright © 2010 by The Korean Orthopaedic Association

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1 — After the terminal deoxynucleotydyl transferase mediated dUTP nick-end labeling (TUNEL) reaction, (A) condensed apoptotic osteocytes in cancellous bone from a femoral head removed from a patient with steroid-induced osteonecrosis show intense green fluorescence (× 400). (B) In a patient with alcohol-induced osteonecrosis, intermediate fluorescence is shown (× 100). Non-apoptotic cells are not visible using immunofluorescence microscopy. (C) In a patient with idiopathic osteonecrosis, no fluorescence is shown (× 100).