FIG. 5. A cellular-membrane permeable version of the DR5 binding domain of c-FLIP triggers apoptosis.

A–C, Jurkat cells were incubated in medium containing vehicle (Mock), 15 µm TAT, or 15 µm TAT-FLIP cellular membrane permeable peptides for 2 h (A and C) or 16 h (B). A, immunofluorescence analysis demonstrated that TAT and TAT-FLIP are internalized. B, cells were analyzed for the percentage of apoptotic sub-G₁ population by flow cytometry. M₁ is the G₀/G₁ apoptotic population. C, percent apoptosis was also determined by annexin V-PE staining and flow cytometry. Apoptotic rates from three independent experiments are shown in B and C.